Objective To study the expression of serum Soluble ST2 (sST2), soluble leucocyte differentiation antigen 40 ligand (sCD40L), growth differentiation factor 11 (GDF11) in patients with chronic heart failure and their correlation with cardiac function. Methods 74 patients with chronic heart failure admitted to our hospital from March 2018 to March 2020 were selected as the subjects of this study, and 65 healthy patients in our hospital were selected as the control group to analyze the correlation between serum sST2, sCD40L and GDF11 and left ventricular ejection fraction (LVEF), anterior and posterior diameter of left ventricle (LAD) and left ventricular end-diastolic diameter (LVEDD). Results The serum levels of sst2, sCD40L, Gdf11, lad and LVEDd in the observation group were significantly higher than those in the control group, and the LVEF level was significantly lower than that in the control group, with significant difference (P＜0.05). The levels of sst2, sCD40L, Gdf11, lad and LVEDd in patients with grade II cardiac function were significantly lower than those in patients with grade III and IV cardiac function, and the LVEF level was significantly higher than those in patients with grade III and IV cardiac function. The levels of sst2, sCD40L, Gdf11 and LAD in patients with grade III cardiac function LVEF, lad and LVEDd were taken as dependent variables, and serum sst2, sCD40L and Gdf11 were taken as independent variables. The results of correlation analysis showed that serum sst2, sCD40L and Gdf11 were positively correlated with LAD and LVEDd (r=0.565, 0.696, 0.756, 0.600, 0.705, 0797, P＜0.05). There was a negative correlation between serum sst2, sCD40L, Gdf11 and LVEF (r=0.513,0.574,0.659, P＜0.05).conclusionIn patients with chronic heart failure, there is a close relationship between the expression of serum sST2, sCD40L and GDF11 and cardiac function, which can promote the disease progression.