Objective To study the effects of long-chain non-coding RNA MIR4435-1HG (LncRNA MIR4435-1HG) on proliferation, migration and invasion of gastric cancer cells and its mechanism. Methods The expression of LncRNA MIR4435-1HG in gastric cancer cell lines (MGC-803, MKN45, AGS) were detected by quantitative polymerase chain reaction (qPCR). AGS cells were divided into control group, siRNA-NC group and siRNA-MIR4435-1HG group. AGS cells in control group were cultured routinely, while the siRNA-NC group and siRNA-MIR4435-1HG group were transfected with siRNA NC plasmid and siRNA MIR4435-1HG by Lipofectamine 2000, respectively. The cell proliferation in each group were detected by Cell counting (CCK-8). The cell migration and invasion were detected by scratch test and Transwell. The apoptosis was detected by flow cytometry. The online database DIANA-LncBase v2, dual-luciferase reporter gene predicted and verified the targeting relationship between LncRNA MIR4435-1HG and microRNA-150-5p (miR-150-5p). Results The expression of LncRNA MIR4435-1HG was up-regulated in gastric cancer cell lines (P＜0.05), and in AGS cells was relatively high. Compared with the control group, the expression of LncRNA MIR4435-1HG in AGS cells of siRNA-MIR4435-1HG group decreased significantly (P＜0.05). Down-regulation of LncRNA MIR4435-1HG inhibited the proliferation of AGS cells (P＜0.05), blocked invasion and migration, and induced apoptosis (P＜0.05). miR-150-5p was the downstream target gene of LncRNA MIR4435-1HG. Conclusion The up-regulation of LncRNA MIR4435-1HG expression in gastric cancer cell lines, and down-regulation of LncRNA MIR4435-1HG may inhibit the proliferation, invasion and migration, and induce apoptosis of AGS cells by targeting miR-150-5p.