Objective The research aimed to study the effect of ropivacaine on growth arrest and oxidative damage of endometrial cancer. Methods The endometrial cancer HEC-1B and Ishikawa cells were selected for research. The CCK-8 method was used to detect the activity of HEC-1B and Ishikawa cells, and finally 1mM ropivacaine was selected to treat HEC-1B and Ishikawa cells. Clone formation test, flow cytometry, Transwell test and microtubule formation test were used to detect cell proliferation, apoptosis, invasion and the number of microtubule formation nodules. Western Blot was used to detect the expression of E-cadherin, Vimentin and VEGF proteins. The expression levels of ROS, 8-OHdG, ATP and mitochondrial superoxide were detected by the kit in HEC-1B and Ishikawa cells. Results Ropivacaine inhibited the proliferation, invasion and microtubule formation of HEC-1B and Ishikawa cells, and promoted cell apoptosis. At the same time, ropivacaine inhibited the expression of Vimentin and VEGF protein, and promoted the expression of E-cadherin protein. In addition, the expression levels of ROS, 8-OHdG and mitochondrial superoxide in HEC-1B and Ishikawa cells were significantly increased, and the expression level of ATP were significantly decreased. Conclusion Ropivacaine could inhibit the proliferation, invasion and microtubule formation of HEC-1B and Ishikawa cells, and promote cell apoptosis and oxidative damage.