【Abstract】 Objective To investigate the effect of inhibiting stomatin-like protein 2 (SLP-2) on migration and invasion of cervical cancer cells. Methods Human cervical adenocarcinoma Hela cells and squamous cell carcinoma Siha cells were divided into siRNA negative control group (siRNA-CON) and SLP2-siRNA group, respectively. Western blot was used to detect the expression of SLP-2 protein in each group after transfection. Transwell lab test was used to detect the invasive ability of cells. Scratch test was used to detect the migration ability of cells. Western blot was used to detect the expression levels of invasive related proteins Rac1, matrix metalloproteinase-2 (MMP-2) and MMP-9. Independent sample t test was used for statistical analysis. Results Western blot results showed that SLP-2 protein levels in Hela cells and Siha cells in SLP2-siRNA group were lower than those in siRNA-CON group, and the difference was statistically significant (P＜0.05). Transwell lab test showed that the invasive ability of Hela cells and Siha cells in SLP2siRNA group was lower than that in siRNA-CON group (P＜0.05). The results of cell scratch test showed that the migration ability of Hela cells and Siha cells in SLP2-siRNA group was lower than that in siRNA-CON group at 24h and 36h (P＜0.05). Western blot results showed that the expression levels of Rac1, MMP-2 and MMP-9 in Hela cells and Siha cells in SLP2-siRNA group were lower than those in siRNACON group (P＜0.05). Conclusion The invasive and migratory abilities of Hela cells and Siha cells of cervical adenocarcinoma decreased significantly after the interference of SLP-2 expression, suggesting that SLP-2 plays an important role in the progression of cervical cancer and may be a potential predictor of metastasis and therapeutic target.