Objective To investigate the regulatory effect of Deferoxamine (DFO) on the expression of hypoxia inducible factor1 (HIF-1)/Vascular endothelial Growth Factor (VEGF), and its promotion effect on spinal cord injury repair. Methods 40 SD rats were modeled after spinal cord injury and randomly divided into 4 groups: sham operation group (10), control group (10), DFO 30mg/Kg intraperitoneal injection group (10) and DFO 100 mg/Kg intraperitoneal injection group (10). The behavioral function and pathological changes of the spinal cord were observed after the operation, and the expression of HIF-1 /VEGF was observed. Results From one week after operation, behavioral function of DFO group and control group was significantly improved (P＜0.05). However, from two weeks after the operation, the behavioral function of rats in the DFO 100 group was further significantly improved compared with the control group (P＜0.01),and the degree of improvement in the DFO 100 group was better than that in the DFO 30 group (P＜0.05). Four weeks after the operation, the spinal cord tissues of rats in the control group were significantly reduced, with only a small amount of HIF1/VEGF expression. The residual area of myelin sheath in the DFO 30 group was significantly restored compared with the control group (P＜0.01), and the residual area of myelin sheath in the DFO 100 group was significantly higher than that in the DFO 30 group (P＜0.05). The HiF1/VEGF expression in DFO 30 group was significantly higher than that in the control group (P＜0.05), and the HIF-1/VEGF expression in DFO 100 group was more significant than that in DFO 30 group(P＜0.05). Conclusion DFO can effectively promote spinal cord injury repair by regulating HiF-1/VEGF expression. Large dose DFO application may have a better effect on spinal cord injury repair.