Objective To investigate the relationship between circular RNA circ-ccnb1 and cyclinb1 and its effect on the development (proliferation and migration) of esophageal cancer.Methods 60 cases of esophageal squamous cell carcinoma and adjacent normal tissues were collected and divided into two groups. The expression of ccnb1 in the two groups was detected by immunohistochemistry, and the correlation between the expression of ccnb1 and clinicopathological factors (lymph node metastasis, pathological stage) was analyzed. Esophageal squamous cell carcinoma cells were cultured and divided into two groups: normal esophageal epithelial cells (Het1-A) and esophageal squamous cell carcinoma cells 109 (Ec109). The expression of ccnb1 protein was detected by Western blot. The cells were divided into three groups: blank group, vector group and cir ccnb1 group. WB was used to detect the change of ccnb 1 protein transferred into the nucleus. Cell proliferation rate (CCK-8 method) and cell migration rate were detected by cell proliferation assay (CCK-8 method) and scratch test. Results The expression level of ccnb1 protein in esophageal squamous cell carcinoma tissues was higher than that in normal tissues (P＜0.05), and the expression level of ccnb1 protein in EC109 cells was higher than that in Het1-A cells (P＜0.05). Compared with the control group, the nuclear translocation level of ccnb1 protein in circccnb1 cells was significantly lower (P＜0.001), and the cell proliferation rate and migration rate were both lower (P＜0.05).Conclusion Circ-ccnb1 can affect the development of esophageal squamous cell carcinoma by inhibiting the nuclear translocation of ccnb1 in esophageal squamous cell carcinoma cells, that is, inhibiting proliferation and migration.