【Abstract】Objective To observe the effect of indocyanine green imaging on preventing anastomotic leakage after laparoscopic colorectal cancer radical operation. Methods 260 patients with colorectal cancer admitted in our hospital from January 2018 to December 2019 were selected as the research object, and they were divided into an observation group (130 cases) and a control group (130 cases) according to the random number table method. The patients in the group were treated with laparoscopic colorectal cancer radical surgery. The patients in the observation group were treated with indocyanine green imaging during laparoscopic colorectal cancer radical surgery to observe the anastomosis blood flow. The operationrelated indicators, the incidence of postoperative anastomotic leakage and the perioperative mortality were compared between the two groups. Results There was no significant difference in the operation time, intraoperative blood loss, postoperative exhaust time, and perioperative mortality between the two groups of patients (P＞005). The observation group had postoperative grade B and C anastomotic leakage. The rate was 153%, which was lower than 692% of the control group, the difference was statistically significant (P＜005); the postoperative hospital stay of the two groups was shorter than that of the control group (P＜005), which was statistically significant. Conclusion Indocyanine green imaging applied to laparoscopic radical resection of colorectal cancer can reduce the incidence of postoperative anastomotic leakage and can be promoted.