Abstract:【Abstract】Objective To investigate the correlation between peripheral blood miR213p, miR223p and acute lung injury (ALI) in patients with severe acute pancreatitis (SAP). Methods Between July 2017 and February 2020, 124 patients with SAP were selected and divided into simple SAP group (78 cases) and SAP with ALI group (46 cases). Meanwhile, healthy volunteers were recruited as the control group. The patients’ peripheral venous samples were collected to detect the expression of miR213p and miR223p by realtime fluorescent reverse transcription quantitative polymerase chain reaction, and clinical data of SAP patients were collected. Results The peripheral blood miR213p and miR223p were significantly higher in the simple SAP group and SAP with ALI group than in the control group (P＜005), and higher in SAP with ALI group than in the simple SAP group (P＜005). Significant differences were found in peripheral blood levels of miR213p and miR223p between patients with different CT grades, patients with and without pleural effusion, and patients with different acute physiology and chronic health examination II (APACHE II) scores (P＜005). The peripheral blood miR213p and miR223p levels of patients with CT grade E, pleural effusion, APACHEⅡ score ≥15 were higher than those of patients with CT grade D, without pleural effusion, and APACHEⅡ score ＜15 (P＜005).Logistic regression analysis showed that CT grade, pleural effusion, and peripheral blood miR213p and miR223p expression levels were associated with ALI in SAP patients (P＜005). The area under the curve (AUC) values of peripheral blood miR213p and miR223p for diagnosing ALI were 0770 and 0812. When the cutoff values were 2668 and 1815, the Youden index was the largest. The AUC of combined diagnosis of the two was 0869. Conclusion Peripheral blood miR213p and miR223p of SAP patients are closely related to the occurrence of ALI, suggesting that their levels can be applied to determine the degree of SAP and the presence or absence of ALI, thereby guiding treatment.
