【Abstract】Objective To explore the effect and molecular mechanism of Lmimosine on proliferation and apoptosis of colorectal cancer cells. Methods Colorectal cancer cells SW480 were divided into control group, low, medium, and highconcentration group of Lmitosine, pcDNA group, pcDNALATS1 group, Lmitosine+siNC group, Lmimosine+siLATS1 group. Methyl thiazolyl tetrazolium assay (MTT) was used to detect the cell proliferation inhibition rate. Western blot method was used to detect Cyclin D1, cyclindependent kinase inhibitor 1A (p21), B cell lymphoma/leukemia2 (Bcl2), Bcl2 related X (Bax), large tumor suppressor gene 1 (LATS1) protein expression; flow cytometry to detect apoptosis. The realtime fluorescence quantitative PCR (RTqPCR ) was used to detect LATS1 mRNA expression. Results In colorectal cancer cells SW480 treated with low, medium and high concentrations of Lmimosaine, the cell proliferation inhibition rate, the apoptosis rate and the expression of LATS1 were increased (P＜005). Overexpression of LATS1 inhibits the proliferation of colorectal cancer cells and promotes apoptosis. Inhibition of LATS1 expression reversed the effect of Lmimosine on proliferation and apoptosis of colorectal cancer SW480 cells. Conclusion LMimosaine may inhibit the proliferation of colorectal cancer cells and promote apoptosis by upregulating the expression of LATS1.