【Abstract】Objective To investigate the effect of pirfenidone (PFD) on proliferation, migration and invasion of cholangiocarcinoma HuCCT1 cells. Methods The proliferation of HuCCT1 cells treated with various concentration（0, 025 g/L, 05 g/L, 075 g/L, 1 g/L）was assessed by CCK8. The effect of PFD on clone ability of HuCCT1 was analyzed by clonal assay. The motility of PFD treatment on HuCCT1 cells was determined by wound healing assay and transwell assay. The expression levels of EMT markers were detected by western blot. Results PFD significantly decreased the proliferation of HuCCT1 cells in a concentrationdependent manner compared with control group（P＜005）. The colony formation was reduced in HuCCT1 cells treated with PFD（P＜005）. The migration and invasion ability of HuCCT1 cells treated with PFD were also inhibited（P＜005）. The results of western blot showed that the expression of Ecadherin was upregulated and the expression of Ncadherin and Vimentin downregulated in PFDtreated cells compared with control group（P＜005）. Conclusion PFD inhibits the proliferation, migration, invasion and EMT process of cholangiocarcinoma HuCCT1 cells.