【Abstract】Objective To investigate the relationship of expression of PDZbinding kinase (PBK) gene and the clinical outcomes of bladder cancer (BC) patients and to provide new markers for diagnosis and treatment. Methods Two independent samples t test, Chisquare test and logrankbased survival analysis were performed using public BC data GSE13507. Moreover, gene set enrichment analysis (GSEA) was applied to study relevant mechanisms. Results Two independent samples t test results suggested that PBK was upregulated in BC cells when compared with normal bladder tissues (P＜0001). Chisquare test indicated that higher expression of PBK was associated with the gender, age, invasiveness, higher grade, T staging, N staging, M staging, disease progression and relapse. BC patients with higher PBK expression had significantly worse overall survival (P=00023) and cancer specific survival (P=00003). GSEA results indicated that PBK may affect the prognosis of BC patients through “MYC targets”, “G2M checkpoint”, “spermatogenesis”, “E2F targets”, “unfolded protein response”. Conclusion PBK expression is correlated with many poor clinicopathological characteristics and prognosis, and it may be a new biomarkers of diagnosis and treatment for BC.