【Abstract】Objective To investigate the effects of conditioning with ChiCGB (chidamide+cladribine+gemcitabine+busulfan) in patients with nonHodgkin's lymphoma (NHL) before autologous hematopoietic stem cell transplantation. Methods The relevant data of 66 patients with NHL admitted to the hospital between March 2015 and July 2019 were retrospectively analyzed. All patients were treated with autologous hematopoietic stem cell transplantation and conditioning with ChiCGB regimen before transplantation. The collection of autologous hematopoietic stem cells, hematopoietic reconstruction after transplantation, regimenrelated toxicity, curative effect and survival were analyzed. Results The medians of collected mononuclear cells and CD34+ cells were 1136×108/kg and 1368×106/kg, respectively. 66 patients successfully completed hematopoietic reconstruction, and the average granulocyte implantation time and average platelet implantation time was (1032±314) d and (1228±706) d, respectively. The incidence of infection during transplantation was 5606%, and the incidence rates of grade 2 or higher grade of bleeding, mucositis, liver injury, diarrhea, fever, nausea and vomiting during transplantation were 758%, 2576%, 2576%, 5606%, 3636% and 6667%, respectively. 46 and 17 patients achieved complete remission and partial remission in 3 months, respectively, and the overall response rate after transplantation was 9545%. The 1year OS rate and PFS rate were 8485% and 7121%. The 3year OS rate and PFS rate were 7273% and 6061%, respectively. Univariate and COX regression analyses showed that disease stage and refractory or recurrent disease were independent risk factors affecting patients’ 3year OS rate (P＜005). Disease stage was an independent risk factor for the 3year PFS rate (P＜005).Conclusion There is no intolerable toxicity caused by conditioning with ChiCGB regimen in patients with NHL before autologous hematopoietic stem cell transplantation. Besides, the prognosis is good. It is a safe and effective conditioning regimen for such patients.