【Abstract】Objective To investigate the effects of gastrodin on the levels of neurobiochemical modulators shared by brain and stomach in a rat model of FD with anxietylike gastric hypersensitivity induced by sequential stress.MethodsRats were sequentially subjected to acute gastric irritation (AGI) and maternal separation (MS) early in life and restraint stress (RS) in adulthood. Then they were given intraperitoneal injection of 0.9% saline (2.0 mL/kg, for control and model group), buspirone (3.125 mg/kg, for buspirone group) or Gastrodin (Gas, 62.5 or 125.0 mg/kg for Gas low or high dose groups), respectively (one time a day at 8:00 o’clock a.m. for 7 consecutive days). After that blood collection were performed and the rats were finally sacrificed by decapitation. The hippocampus and gastric fundus were promptly isolated and stored at 80 ℃ for ELISA measurements of 5hydroxytryptamine (5HT), γaminobutyric acid (GABA), brainderived neurotrophic factor (BDNF) and nesfatin1.Results Compared with the control group, the hippocampal, plasmic and gastric levels of 5HT, BDNF and GABA decreased significantly (P＜001), while the concentrations of nesfatin1 increased greatly (P＜001). Gas could significantly attenuate the diminution of the hippocampal, plasmic and gastric levels of 5HT, BDNF and GABA, but inhibit the increase of nesfatin1 in the same sites (P＜001 vs. model rats). Conclusions The results implied that Gas could influence the levels of 5HT, GABA, BDNF and nesfatin1 in hippocampus, plasma and gastric tissue of the sequentialstresstreated rats, and could be used in the treatment of FD with anxietylike gastric hypersensitivity.