【Abstract】 Objective To investigate the effects of lncRNA DLX6-AS1 on proliferation, migration, invasion and apoptosis of cervical cancer cells and its mechanism. Methods The normal cervical cells (ect1/E6E7) and cervical cancer cell lines HeLa, SiHa, c33a and CaSki were cultured. SiHa was randomly divided into control (NC) group, Si-CON group, Si-dlx6-AS1 group, pcDNA group, pcDNA-dlx6-AS1 group, miR-CON group, miR-103a-3p group, Si-dlx6-AS1+anti-miR-CON group, Si-dlx6-AS1+anti-miR-103a-3p group. The expression levels of miR-103a-3p and dlx6-AS1 were detected byRT-qPCR. Western blot was used to detect the protein expression. MTT assay was used to detect cell viability and flow cytometry was used to detect apoptosis. Transwell was used to detect cell migration and invasion. Double luciferase reporter assay was used to detect the targeting relationship between dlx6-AS1 and miR-103a-3p. Results Compared with the normal cervical cell ect1/e6e7, the expression level of dlx6-AS1 in HeLa, SiHa, c33a and CaSki increased, and the expression level of miR-103a-3p decreased, and the difference was statistically significant (P＜0.05). After interfering with dlx6-AS1 expression or miR-103a-3p overexpression, the cell activity decreased, apoptosis rate increased, migration and invasion decreased, CyclinD1, BCL-2, MMP-2, MMP-9 expression decreased, Bax and cleaved caspase-3 were increased, and the difference was statistically significant (all P＜0.05). After inhibiting the expression of miR-103a-3P and dlx6-AS1, the expression levels of MMP-2, MMP-9, CyclinD1 and Bcl-2 in SiHa were increased, the expression levels of Bax and cleaved caspase-3 decreased, cell activity increased, apoptosis rate decreased, and the difference was statistically significant (all P＜0.05). Dlx6-AS1 targets the expression of miR-103a-3p. Conclusion Interfering the expression of DLX6-AS1 can inhibit the proliferation, migration and invasion of cervical cancer cells and promote apoptosis. The mechanism may be related to the expression of miR-103a-3p, which will provide new ideas and targets for the treatment of cervical cancer.