Objective To analyze the clinical significance of serum troponin T (cTnT), myoglobin (MYO), creatine kinase isoenzyme (CK-MB) and N-terminal probrain natriuretic peptide (NT-proBNP) levels in patients with congestive heart failure (CHF). Methods From June 2016 to June 2019, 95 cases of CHF patients in our hospital were selected as the observation group, and 30 healthy volunteers in our hospital during the same period were selected as the control group. The serum levels of cTnT, MYO, CK MB and NT proBNP were compared between the two groups. The serum levels of cTnT, myo, CK MB and NT proBNP in CHF patients with different heart failure grades, different NYHA cardiac function grades and different prognosis (death and survival) were analyzed. The ROC curve was drawn to analyze the diagnostic value of Serum cTnT, MYO, CK MB and NT proBNP levels in CHF patients.Results The serum levels of cTnT, MYO, CK MB and NT proBNP in the observation group were higher than those in the control group (P＜0.05). The levels of cTnT, MYO, CK MB and NT proBNP in patients with heart failure grade CD were higher than those in patients with heart failure grade AB (P＜0.05). With the increase of NYHA cardiac function grade, the levels of cTnT, MYO, CK MB and NT proBNP in CHF patients increased (P＜0.05). The serum levels of cTnT, MYO, CK MB and NT-proBNP in the dead were higher than those in the survivors (P＜0.05). The ROC curve analysis showed that the sensitivity, specificity, Youden index and area under ROC curve of combined detection of Serum cTnT, myo, CK MB and NT proBNP in the diagnosis of CHF were higher than those in single diagnosis. Conclusion The combined detection of cTnT, MYO, CK MB and NT-proBNP has high diagnostic value for CHF. CTnT, MYO, CK MB, NT-proBNP are also closely related to the prognosis of CHF patients, and should be taken as the key monitoring indicators of CHF.