Objective To investigate the expression of long noncoding RNA actin filament associated protein 1 antisenseRNA1 (AFAP1-AS1) in NSCLC and normal lung tissue, and analyze the relationship of long noncoding RNA AFAP1AS1 with clinicpathological features in NSCLC.Methods The expression levels of long noncoding RNA AFAP1AS1 in NSCLC and normal lung tissue were detected by using realtime reverse transcriptionpolymerase chain reaction(RT-qPCR), and their correlation with clinicpathologic features of NSCLC tumor was analyzed. 〖WTHZ〗Results 〖WTBZ〗Expressionlevels of long noncoding RNA AFAP1AS1 were significantly higher than that in NSCLC tissues in normal lung tissues. The expression of long noncoding RNA AFAP1-AS1 was associated with tumor size, TNM stage and lymph node metastasis. The expression of long noncoding RNA AFAP1AS1 was higher in NSCLC tissues of patients whose tumor size was larger than 3cm, or who had late TNM stage or who appeared lymph node metastasis. Conclusion The levelof long noncoding RNA AFAP1-AS1 expression is higher in NSCLC than that in normal lung tissues. It may be an oncogene in NSCLC.