Objective To investigate the inhibitory effect of Allicin on apoptosis of deep second degree burn wounds in rats and explore its mechanism.Methods 150 experimental Wistar rats were randomly divided into pseudoburn group, model group, and Allicin low, medium and highdose group (5, 10, and 20 mg/kg), n=30. The model rats with deep II degree burn injury were made by using a pressure of 003MPa and 106 ℃ for 5 seconds. 1 time/d intraperitoneal injection (sham burn group, model group were given 0.9% sodium chloride solution) for 14d. The burn wounds of rats in each group were observed and HE staining pathological examination. The burn wound healing rate was calculated. The cell apoptosis was observed by TUNEL method and calculate Apoptosis Index (AI), the expression of bcl-2, Bax, PI3K, p-Akt, NF-B were detected by Western blot. The activity of SOD, CAT and the content of MDA were detected by biochemical analysis. Results Compared with the model group, the wounds of rats in various doses of Allicin showed different degrees of shrinkage, redness of the newly healed skin, and pathological morphological changes were improved to varying degrees. The wound healing rate increased in Allicin medium and highdose groups. The number of apoptotic cells in burn wound tissue increased significantly and the AI significantly decreased. The expression of bcl2, Bax, PI3K, pAkt were significantly upregulated and NF-B was significantly downregulated. The activity of SOD, CAT were significantly increased and the content of MDA was significantly decreased (P＜0.05). Conclusion Allicin canpromote the healing of deep seconddegree burn wounds in rats by inhibiting apoptosis, which may be related to regulating apoptosisrelated protein expression, activating the PI3K/Akt signaling pathway and inhibiting oxidative stress.