【Abstract】Objective To investigate the effect and potential mechanism of umbilical cord mesenchymal stem cells (UCMSC) in the treatment of diabetic foot. Methods 86 patients with diabetic foot diagnosed and treated from February 2017 to June 2019 were selected as the study objects. According to the random number table method, they were divided into control group and observation group, 43 cases in each group. Patients in the control group were treated with conventional therapy, while patients in the observation group were treated with intravenous injection and local injection of UCMSC. The foot sign score, Wagner grade, serum inflammation index, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell derived factor1α (SDF1α) levels and total effective rate of the two groups were compared before and after treatment. Results The skin temperature, pain degree, skin color, ankle brachial index and intermittent lameness score of the foot in the observation group were significantly higher than those in the control group (P＜005). Wagner grade and serum levels of tumor necrosis factorα (TNFα), interleukin6 (IL6), high sensitivityC reactive protein (hsCRP) in the observation group were significantly lower than those in the control group (P＜005). The serum levels of VEGF, bFGF and SDF1α in the observation group were significantly higher than those in the control group (P＜005). The total effective rate in the observation group was significantly higher than that in the control group. Conclusion Intravenous injection combined with local injection of umbilical cord mesenchymal stem cells has a significant effect on diabetic foot, and the mechanism of action is related to antiinflammatory and promoting angiogenesis.