Objective The aim of this study was to investigate the association of growth arrest specific protein 6(GAS6) gene polymorphism and plasma level with type 2 diabetes mellitus complicated with atherosclerosis（T2DMAS） in Kunming Han population.Methods Peripheral venous blood was collected from 181 patients with type 2 diabetes, 171 patients with T2DMAS and 186 normal controls. Rs8191974 polymorphism was detected by PCR and unidirectional gene sequencing. The plasma GAS6 level was detected by ELISA, and the genotype frequency, allele frequency, plasma GAS6 level and related clinical indexes were analyzed.Results There was no difference in the frequency distribution of the three genotypes between T2DM group and NC group, T2DMAS (2=8031, P=0018；2=1762，P=0414 ). The frequency of GG genotypes in the T2DMAS group was higher than that in the NC group，but the frequency of GA and AA genotype was lower (2=9286, P=0010).（2）Compared with NC group, the frequency of allele A was lower, but allele G was higher in T2DM and T2DMAS groups (2=7493,P=0006;2=8718，P=0003). There was no difference in allele frequency between T2DMAS and T2DM group(2=0069，P=0793). Compared with NC group, the levels of Body Mass Index (BMI), systolic blood pressure (SBP ), diastolic blood pressure (DBP), total cholesterol (TC), triglycerides(TG), low density lipoprotein cholesterol(LDLC) and fasting plasma glucose（FBG）were higher (P＜001), but the levels of highdensity lipoprotein cholesterol (HDLC) and GAS6 were lower in T2DM and T2DMAS group (P＜001). Compared with T2DM group, the course of T2DM,SBP and intima media thickness( IMT) were higher in T2DMAS group (P＜001), but there was no difference in GAS6 level between T2DM and T2DMAS group (P＞0.05）. The course of T2DM, alcohol consumption and SBP are the risk factors of AS（OR:1072, 2625, 1019; 95% CI:10241122,12415552,10031035）. HDLC is a protective factor for AS（OR：0253；95% CI：00710902.However, GAS6 gene rs8191974 polymorphism and plasma level may not be associated with AS. Conclusion The association of GAS6 gene rs8191974 polymorphism and plasma level with AS has not been found in Kunming Han population. The course of T2DM, alcohol consumption and hypertension are risk factors for AS. HDLC is a protective factor for AS.