Objective To study the regulatory effect of longchain noncoding RNA (lncRNA) DLEU2 on microRNA (miR) 4103p and its effect on the proliferation, migration and invasion of rheumatoid arthritis (RA) synovial fibroblasts. Methods qRTPCR was used to detect the expression of DLEU2 and miR4103p in the synovial tissue of patients with rheumatoid arthritis. LncBase Predicted v.2 prediction and dual luciferase reporting experiments analyzed the targeting relationship between DLEU2 and miR4103p. Transfected siDLEU2 or miR4103p, or cotransfected siDLEU2 and antimiR4103p in RA synovial fibroblasts MH7A. MTT, Transwell, and Western blot were applied to determine the proliferation, migration, invasion and expression of CyclinD1, p21, Matrix metalloprotease (MMP)2, and MMP9 in RA synovial fibroblasts MH7A. Results The expression of DLEU2 in synovial tissue of RA patients increased significantly, and the expression of miR4103p decreased dramatically (P＜0.05). lncRNA DLEU2 targeted the regulation of miR4103p expression. Inhibition of DLEU2 expression or miR4103p overexpression obviously reduced OD values of RA synovial fibroblasts MH7A at 24 h, 48 h and 72 h, migrating cells, invasive cells, levels of CyclinD1, MMP2, and MMP9 proteins, but evidently enhanced the expression level of p21 protein (P＜0.05). Interfering with the expression of miR4103p reversed the inhibition of DLEU2 expression on the proliferation and migration of RA synovial fibroblasts MH7A. Conclusion lncRNA DLEU2 is upregulated in rheumatoid arthritis synovial tissues, and inhibition of its expression inhibits the proliferation, migration and invasion of rheumatoid arthritis synovial fibroblasts by targeting miR-410-3p.