Objective To study the protective effect of emodin on myocardial injury in sepsis rats. Methods The rats were divided into control group, model group (sepsis rat model), lowdose group (sepsis rat model, 30 mg/kg emodin by gavage), medium dose group (sepsis rat model, 60 mg/kg emodin by gavage), highdose group (sepsis rat model, 90 mg/kg emodin by gavage),10 in each group. The levels of MDA, SOD and GSH PX in myocardial tissue were detected by the kit. The apoptosis of cardiomyocytes was detected by TUENL method. The activity of caspase 3 in myocardium was detected by kit. Results Compared with the control group, MAP and LVSP in the model group were reduced, LVEDP and serum myocardial enzymes were increased, TNFα, IL1β, and IL6 mRNA levels were increased in myocardial tissue, SOD and GSHPX activities were decreased, and MDA levels were increased, cardiac cell apoptosis rate and CCaspase3 activity increased. Compared with the model group, MAP and LVSP of rats in the lowdose group, mediumdose group, and highdose group increased sequentially, LVEDP and serum myocardial enzymes decreased in turn, TNFα, IL1β, and IL6 mRNA levels in myocardial tissue decreased, SOD, GSHPX activity increased in turn, MDA level decreased in turn, myocardial apoptosis rate and CCaspase3 activity decreased in turn. Conclusion Emodin exerts protective effects on myocardial injury in sepsis rats by reducing myocardial oxidative stress, apoptosis and inflammation.