Abstract:Objective To investigate the myocardial protective effect of ligoliptin in regulating autophagy pathway in diabetic rats with myocardial ischemia reperfusion injury. Methods 40 SD rats aged 8 weeks were divided into sham operation group, diabetes group, model group and treatment group. After successful modeling, ligoliptin was injected into the treatment group through the caudal vein, and normal saline of equal volume was injected into the other three groups. The body weight and blood sugar for 3 days were Monitored. Venous blood samples were taken 3 days later for aspartate aminotransferase, creatine kinase, creatine kinase isozyme and lactate dehydrogenase. Histopathological changes of myocardial cells in each group were compared by HE staining. The expression of autophagy related genes was detected by fluorescence quantitative PCR and westernblot. Results On the second and third day, the body weight of the sham operation group and the treatment group gradually increased, and the weight of the diabetes group and the model group decreased gradually. The blood glucose concentration of the diabetes group and the model group was higher than that of the sham operation group and the treatment group. The levels of AST, CK, CK MB and LDH in sham operation group, diabetes group and treatment group were lower than those in model group (P < 0.05). The results of quantitative PCR and Western blot showed that the expression levels of Akt and mTOR in the sham operation group and the treatment group were lower than those in the diabetic group and the model group (P < 0.05).Conclusion Ligoliptin can regulate the relevant genes in the autophagy pathway in rats, which is beneficial to the improvement of diabetes mellitus, and plays a protective role in the myocardium of rats with myocardial ischemia reperfusion injury.
