Objective To investigate the effects of microRNA933p (miR933p) on high glucoseinduced renal tubular epithelial damage, inflammatory factor secretion and PI3K/AKT signaling pathway. Methods Renal tubular epithelial cells were cultured in vitro and divided into NC group, HG group, antimiRNC group, antimiR933p group, HG+ miRNC group, HG+miR933p group.Renal tubular epithelial cells were cultured in vitro, and the experimental groups were: NC group, HG group, antimiRNC group, antimiR933p group, HG+ miRNC group, HG+miR933p group. The expression of miR933p was detected by realtime fluorescent quantitative polymerase chain reaction (qRTPCR). The levels of TNFα and IL6 were detected by enzymelinked immunosorbent assay (ELISA). MTT was used to detect cell proliferation ability. Apoptosis rate was measured by flow cytometry. The expression levels of CyclinD1, Ccaspase3, pPI3K and pAKT were detected by Western blot. Results Compared with the NC group, the expression level of miR933p was significantly decreased in the HG group (P＜0.05), the cell survival rate was significantly decreased (P＜0.05), and the expression level of CyclinD1 was significantly decreased (P＜0.05), but the apoptosis rate was significantly increased (P＜0.05), the expression levels of Ccaspase3, pPI3K and pAKT were significantly increased (P＜0.05), and the levels of TNFα and IL6 were significantly increased (P＜0.05). ＜0.05). Compared with the antimiRNC group, the cell survival rate of the antimiR933p group was significantly decreased (P＜0.05), and the expression level of CyclinD1 was significantly decreased (P＜0.05), but the apoptosis rate was significantly increased (P＜0.05), the expression level of Ccaspase3 was significantly increased (P＜0.05), and the levels of TNFα and IL6 were significantly increased (P＜0.05). Compared with HG+ miRNC group, the survival rate of HG+miR933p group was significantly increased (P＜0.05), the apoptosis rate was significantly decreased (P＜0.05), and the expression level of CyclinD1 was significantly increased (P＜0.05). ＜0.05), the expression levels of Ccaspase3, pPI3K and pAKT were significantly decreased (P＜0.05), and the levels of TNFα and IL6 were significantly decreased (P＜0.05). Conclusion miR933p inhibits high glucoseinduced renal tubular epithelial cell damage and inflammatory factor secretion by regulating PI3K/AKT signaling pathway.