【Abstract】 Objective To study the effects of miR-122 on the proliferation, epithelial mesenchymal transition (EMT) and receptor tyrosine kinase (AXL)/hypoxiainducible factor1α (HIF-1α) signaling pathway of endometrial carcinoma cell line (RL-952). Methods Human endometrial carcinoma cell line RL-952 was cultured in vitro, and the cells were divided into blank control group (culture medium only), negative control group (miR-122 NC transfection) and miR-122 mimics group (miR-122 mimics transfection). The level of miR-122 was detected by realtime fluorescent quantitative PCR (qRT-PCR). The cell proliferation was detected by human cholecystokinin/cholecystokinin octapeptide (CCK-8) kit. The apoptosis was detected by flow cytometry. Transwell assay was used to detect cell migration and invasion. The expressions of AXL, HIF-1α, E-cadherin, α-Catenin, N-cadherin and vimentin proteins were detected by Western blot. Results Compared with blank control group and negative control group, the cell proliferation, cell migration, cell invasion, the expressions of AXL, HIF-1α, N-cadherin and vimentin proteins in RL-952 cells in miR-122 mimics group showed a significant decrease after 48h of transfection (P＜0.05), the expressions of E-cadherin, α-Catenin proteins and apoptosis rate were significantly increased (P＜0.05). There was no significant difference in cell proliferation, cell migration, cell invasion, apoptosis, expressions of AXL, HIF-1α, E-cadherin, α-Catenin, N-cadherin and Vimentin between the blank control group and the negative control group (P＞0.05). Conclusion Overexpression of miR-122 can inhibit the proliferation, migration, invasion and epithelial stromal transformation of endometrial cancer cells, and promote cell apoptosis, which may be achieved by inhibiting the AXL/HIF-1α pathway.