【Abstract】 Objective To investigate the effect of downregulating of microRNA-197 (miR-197) on the invasion and metastasis of colon cancer HCT116 cells and its mechanism. Methods HCT116 cells were randomly divided into miR-197 antisense oligonucleotide (inhibitor) group, negative control (NC) group and blank control (Mock) group. The miR-197 antisense oligonucleotide (miR-197 inhibitor) was transfected into HCT116 cells by liposome mediated transfection. The expression of miR-197 was determined by qRT-PCR. The target gene of miR-197 was predicted by bioinformatics, and the expression of CD82 protein was detected by Western blot. The invasion and migration ability of HCT116 cells was detected by transwell cell experiment. Results After transfection into HCT116 cells with miR-197 inhibitor, the expression of miR-197 in the inhibitor group was significantly lower than that of NC group and Mock group (P＜0.05). After downregulating the expression of miR-197, the expression of CD82 protein in the inhibitor group was significantly higher than that of other two groups (P＜0.05). The invasion and migration ability of HCT116 cells transfected with miR-197 inhibitor was lower than that of NC group and Mock group (P＜0.05). Conclusion Downregulation of miR-197 in colon cancer HCT116 cells can inhibit invasion and metastasis of tumor cells, and the mechanism may be related to increased expression of the target CD82, which will provide a new target for the treatment of colon cancer.