【Abstract】 Objective To investigate the effect of miR-208a-3p targeting LZTFL1 on the proliferation, invas cancer cells by targeting LZTFL1 ion and metastasis of cervical cancer cells and its molecular mechanism. Methods From May 2016 to March 2018, surgical specimens of 40 patients with cervical cancer were collected from Department of gynecology and obstetrics, Hubei provincial hospital of integrated traditional Chinese & Western medicine. The expressions of miR208a3p and LZTFL1 in cervical cancer tissues and their adjacent tissues were detected by realtime fluorescence quantitative PCR (RT-qPCR) and Western Blot. StarBase online prediction, luciferase reporter gene experiments and Western Blot were used to verify the targeting relationship between miR208a3p and LZTFL1. Hela cells were divided into anti-miR-NC, anti-miR-208a-3p, pcDNA, pcDNA-LZTFL1, anti-miR-208a-3p+si-NC, anti-miR-208a-3p+si-LZTFL1 groups. Cell viability was measured by MTT method. The number of cell migration and invasion was measured by the Transwell test. The expression of CyclinD1, MMP2 and MMP9 proteins was detected by Western blot. Results Compared with adjacent cancer tissues, the expression of miR-208a-3p was significantly increased in cervical cancer tissues, while the expression of LZTFL1 was significantly decreased (P＜0.05). miR-208a-3p targets LZTFL1 and negatively regulates its expression. Compared with the anti-miR-NC group, cell viability, migration and invasion ability, expression of CyclinD1, MMP-2 and MMP-9 protein of Hela cell in anti-miR-208a-3p group was significantly reduced, whereas the expression of p21 was significantly increased (P＜0.05). Compared with the pcDNA group, cell viability, migration and invasion ability, expression of CyclinD1, MMP-2 and MMP-9 protein of Hela cell in pcDNA-LZTFL1 group was significantly reduced, whereas the expression of p21 was significantly increased, the difference was statistically significant(P＜0.05). Compared with anti-miR-208a-3p+si-NC group, cell viability, migration and invasion ability, expression of CyclinD1, MMP-2 and MMP-9 proteins of Hela cell in anti-miR-208a-3p+si-LZTFL1 group were significantly increased, whereas the expression of p21 was significantly reduced, and the difference was statistically significant (P＜0.05). Conclusion miR-208a-3p is highly expressed and LZTFL1 is lowly expressed in cervical cancer. Inhibition of miR-208a-3p could inhibit the proliferation, migration and invasion of cervical cancer cells by upregulating LZTFL1.