【Abstract】 Objective To investigate the mechanism of miR-26a on its activity and Wnt signaling pathway in human hepatoma HepG2 cells. Methods The human liver cancer cell line HepG2 cells were divided into liver cancer group (HepG2 cells without intervention), miR-26a group (intervention of HepG2 cells with miR26a mimics) and miR-26a-NC group (using miR-26aidling HepG2 cells). Transwell cells and scratches were used to detect their invasion and migration ability. The apoptosis was detected by flow cytometry. Results There was no significant difference between miR-26a-NC group and HCC group in the number of invasion, migration rate and apoptosis rate of HepG 2 cells (P＞0.05). Compared with miR-26a-NC group, the invasion number, migration rate and apoptosis rate of HepG 2 cells in miR 26a group were significantly different from those in miR-26a-NC group (P＜0.05). There were significant differences in the levels of β-catenin and Wnt 3 between miR-26a-NC group and hepatoma group (P＞0.05). There was no significant difference in the levels of β-catenin and Wnt 3 between miR-26a-NC group and miR 26a NC group (P＜0.05). There was no significant difference in the mRNA levels of β-catenin and Wnt 3 between miR-26a-NC group and hepatoma group (P＞0.05). The mRNA levels of β-catenin and Wnt 3 in HepG2 cells of miR-26a group were significantly higher than those in miR-26a NC group (P＜0.05). Conclusion Transfection of miR-26a mimics in liver cancer cells can inhibit cancer cell migration and invasion, accelerate apoptosis. The mechanism may be related to the inhibition of β-catenin and Wnt3 levels.