Objective The aim of this study was to observe on the repair effect of rabbits’ degenerative intervertebral disc on early stage with allogeneic nucleus pulposus cell (NPC) transplantation. Methods NPC was isolated and cultured in vitro, and identified with histochemical staining, immunochemical staining and Immunofluorescence staining. The intervertebral discs degeneration animal model was constructed by the conventional punctured method and at the same time NPC was transplanted into the rabbit’s model of disc degeneration. Twentyseven 5monthold Japanese white rabbits were randomly divided into 3 groups. Health intervertebral disc group was set as the normal control (NC) group（n=9）. The NPC transplantation group was set as the positive group. Dulbecco’s modifed Eagle’s medium (DMEM) injection group was set as the negative group. L3-4、L4-5 and L5-6 intervertebral discs were set as samples of experiment in every rabbits. In 4, 8 and 12 weeks after the transplantation, Repair effect of rabbits’degenerative intervertebral discs were evaluated by standard T2weighted image ratio (%ST2WI), histology, degeneration score, immunochemical staining, Immunofluorescence staining, and mRNA expression levels of collagen II and aggrecan. Results In 12 weeks after celltransplantation, standard T2weighted image ratio (%ST2WI), mRNA expression levels of collagen II and aggrecan for DMEM group were signifcantly lower than that of DMEM group (P＜0.05). While no significant differences between the NC and NPC groups were found. Histological analysis indicated that the degeneration score for the NC and NPC groups was signifcantly lower than the DMEM group (P＜0.05). There was no significant difference between NC and NPC groups. Immunofluorescence staining of Collagen Ⅱ and aggrecan indicated obviously positive in NC and NPC groups, and that of DMEM group gradually weakened with the change of time. Conclusion NPCs has a positive effect on restraining the discs degeneration.