Objective To explore the promoting effect of Panlongqi Tablet on the healing of tibial fracture in rats and its possible mechanism. Methods The tibial fracture model rats were established and divided into Model group, PanlongqiTablet group (Treat group), β-catenin inhibitor group (XAV939), treat + XAV939 group, with 10 rats in each group. The healing of tibial fracture was observed by Xray. Bone mineral density (BMD) of tibia was measured by dual energy xray absorptiometry. HE staining was used to observe the structural changes of fracture site. ALP, bFGF, TNFα and IL1β in serum were detected by ELISA. Western blotting was used to detect the protein expressions of pglycogen synthetase kinase 3β (GSK-3β), Wnt3, β-catenin and lymphoid enhancer 1 (LEF1) in bone tissue.ResultsCompared with Sham group, the healing score of bone tissue imaging, BMD, the levels of ALP, bFGF, and the protein expressions of Wnt3, β-catenin and LEF1 in Model group were decreased, and the levels of TNFα and IL1β increased. Compared with the Model group, the healing score of bone tissue imaging, BMD, the levels of ALP, bFGF, and the protein expressions of Wnt3, β-catenin and LEF1 in Model group were increased, and the levels of TNFα and IL1β -decreased.The healing score of bone tissue imaging, BMD, the levels of ALP, bFGF, and the protein expressions of Wnt3, β-catenin and LEF1 in Treat + XAV939 group were higher than those in XAV939 group, and the levels of TNFα and IL1β were lower than those in XAV939 group (P＜0.05). Conclusion Panlongqi Tablet can promote the healing of tibial fracture in rats. Its mechanism is related to the inhibition of Wnt/β-catenin pathway and the alleviation of inflammatory response in rats.