Objective To synthesis 11C-methionine (MET) in a rapid and automatic way, then perform 11C-MET PET/CT imaging in glioma patient. Methods We rapidly synthesized 11C-MET based on CFN-MPS-200 automatic synthesis module, and detected the radiochemical purity by HPLC and TLC. Then 11CMET was injected into glioma patient for PET/CT imaging. Results The whole synthesis procedure consumed about 11 min. The radio chemical yield was about 82±5% (after attenuation correction, n=3) and radio chemical purity was more than 99%. The product was colorless and clear solution with pH value of 7.08.0. The pyrogenic reaction test and bacteria test of product was negative. 11C-MET showed higher tumor/nontumor ratio in glioma patient than 18FFDG. Conclusion Based on CFN-MPS-200 module, we could rapidly synthesize 11C-MET with high radio chemical purity. 11C-MET showed higher tumor/nontumor ratio in glioma patient, it could differentiate tumor area and normal brain tissue clearly.