Objective To explore the effects of reduced glutathione combined with highflux hemodialysis on serum cystatin C (Cys-C), kidney injury molecule1 (KIM-1) and creatinine (SCr) in patients with acute kidney injury (AKI). Methods 6 patients were randomly divided into control group (38 cases) and observation group (38 cases). All patients were given comprehensive treatment. The control group was given highflux hemodialysis. The observation group was given reduced glutathione based on the above treatment. The clinical efficacy, oliguria duration, polyuria duration and serum creatinine recovery time, and serum kidney injury biomarkers before treatment and after 7d of treatment were compared between the two groups. The treatment safety was evaluated in the two groups. Results The total clinical effective rate in observation group was significantly higher than that in control group (84.21% vs 63.16%, P＜005). The oliguria duration, polyuria duration and serum creatinine recovery time in observation group were significantly shorter than those in control group (P＜005). After 7d of treatment, the levels of serum CysC, KIM1, SCr and ALR were significantly decreased in the two groups, and the changes in observation group were significantly greater than those in control group. There were no statistically significant differences in the total incidence rate of adverse reactions and 30d mortality rate between observation group and control group (13.16% vs 7.89%, 2.63% vs 7.89%, P＞005).Conclusion Reduced glutathione combined with highflux hemodialysis has exact efficacy in the treatment of AKI, and it 〖JP2〗can significantly improve the renal function, shorten the oliguria duration, polyuria duration and serum creatinine recovery time, and it has good safety in clinical application.