Objective To analyze the results of combined detection of serum tumor markers (TM) and bone marrow morphology in lymphoma. Methods The clinical data of 70 patients with lymphoma received treatment in the Hospital from March 2017 to March 2019 were selected and set as the study group. The clinical data of 70 healthy subjects were selected during the same period as the control group. The bone marrow morphology examination was carried out in both groups. The electrochemiluminescence was used to detect the serum tumor markers, and the detection results in the two groups were analyzed. Results CA125, CA15 3, CEA and SF in the observation group were higher than those in the control group (P < 0.05). In the observation group, CA125, CA15 3, CEA, SF, WBC and lymphoid cells were higher in infiltrating group than in non infiltrating group, and PLT was lower in infiltrating group than in non infiltrating group (P < 0.05). Conclusion Combined detection of serum tumor markers and bone marrow morphology in patients with lymphoma can be used to diagnose the disease and condition of patients with lymphoma, and to evaluate whether bone marrow infiltration is carried out, so as to select a reasonable clinical treatment plan, which has a certain clinical application value. 