Objective To investigate the impact of ischemia reperfusion injury on the TNFα/IL-6 /STAT-3 pathway in the remnant liver after subtotal hepatectomy models. Methods 60 male SD rats weighting 230～280g were obtained and randomly divided into groupⅠ (subtotal hepatectomy, SH), group Ⅱ (ischemia reperfusion, IR). The left and middle liver was resected, and the remnant liver was treated as followed: the blood flow was not blocked in SH group, but blocked 30 minutes in IR group, then reperfused. After 1, 6, 12, 24 and 48h, respectively, the serum TNFα, IL-6 was detected. The mRNA of CyclinD1, Cdk4 and STAT3 were assayed. The protein expression of CyclinD1 and Cdk4 were detected. Results Compared with SH group, the expression of IL6 declined at each indicated time point, but the content of TNFα increased in early stage(1～12h) in IR group. Moreover, the expression of CyclinD1, Cdk4 and STAT3 was significant decrease in IR group than that in SH group in 24h and 48h. Conclusion Ischemia reperfusion injury could reduce the expression of TNF/il-6 /STAT-3 signaling pathway in the residual liver tissue after subtotal hepatectomy, and may ultimately inhibit the proliferation of hepatocytes.