【Abstract】Objective To study the molecular mechanism of hyacinth bletilla/micron pseudoginseng cream on inflammatory microenvironment of diabetic foot ulcers in rats. Methods Hyacinth bletilla /micronpseudoginseng cream was prepared. 32 SPF grade healthy SD rats were randomly divided into four groups, 8 rats in each group. The control group was normally feed. The other three groups were given different concentrations of hyacinth bletilla/micron pseudoginseng cream including low dose group (5 μ mol/L), middle dose group (10 μ mol / L) and high dose group (20 μ mol/L). The administration was repeated twice on the 7th day with an interval of 1 h. At 30 minutes after the last administration, the serum containing drugs was prepared by aseptic operation, chloral hydrate anesthesia and blood collection from abdominal aorta. RAW264.7 was induced by LPS to establish a cell injury inflammation microenvironment model (LPS stimulation group), which was verified by ELISA. MTT method was used to analyze the cytotoxicity on RAW264.7. TNF α and IL 6 were detected by ELISA, and Griess method was used to detect the cytokines NO secretion was detected.Results The serum containing hyacinth bletilla/micron pseudoginseng has the related blood concentration, which meets the basic requirements of acting as a cell drug. The inflammatory model has a significant increase in TNF α and IL 6 compared with the normal cultured cells (P＜005). The serum containing hyacinth bletilla/micron pseudoginseng (5-40 μmol/L) has no significant toxicity to RAW264.7 cells (P＞005). The serum containing drugs with different concentrations has no significant toxicity to RAW264.7 cells (P＞005). TNF α and IL 6 in the model of inflammatory cell injury were statistically significant (P＜005). Compared with LPS stimulation group, no secretion in different concentrations of serum were significantly reduced (P＜005). Conclusion Hyacinth bletilla/micronpseudoginseng containing serum inhibits TNFα, IL6 and NO in a model of cellular inflammatory injury, which can reduce local inflammatory response and vascular and nerve damage, improve local tissue immunity, and improve the inflammatory microenvironment and promote the wound healing.