【Abstract】 Objective To investigate the effect of ropivacaine intraperitoneal spray and incision infiltration on pain mediators in patients with cervical cancer. Methods 142 cases of cervical cancer admitted from May 2016 to may 2018 were divided into control group (63 cases) and study group (79 cases). The patients in control group were given ropivacaine incision infiltration after operation, and the patients in study group were given ropivacaine incision infiltration and intraperitoneal spray after operation. The pain scores (NRS) , prostaglandin E2 (PGE2), substance P (SP) andβ- endorphin (β-EP) and adverse reactions were compared between the two groups at different time points. Results There was no significant difference in NRS score between the two groups before operation (P>0. 05). At 6h and 12h after operation, the static and dynamic NRS scores of the study group were lower than those of the control group, the difference was statistically significant (P＜0. 05). There was no significant difference in PGE 2, SP and β-EP between the two groups before operation (P>0. 05). 12 hours after the operation, the levels of PGE 2 and SP in the two groups increased, while the level of p EP in the two groups decreased. The changes in the study group were more obvious, and the difference was statistically significant (P＜0. 05). There were vertigo, nausea and vomiting in both groups. There was no significant difference in the incidence of total adverse reactions between the two groups (P>0. 05). Conclusion Ropivacaine intraperitoneal spray and incision infiltration can significantly reduce postoperative pain in patients with cervical cancer, regulate the expression of pain mediators, and analgesia is more safe.