【Abstract】 Objective To investigate the expression and clinical significance of LncRNA SLC7A11-AS1 in human gastric cancer. Methods 78 pairs of human gastric cancer were gathered from January 2016 to June 2018 in The Affiliated Hospital of North Sichuan Medical College. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of SLC7A11-AS1 in 3 gastric cancer cell lines and 1 normal gastric mucosal cell line, gastric cancer tissues and adjacent tissues. Results The relative expression of SLC7A11-AS1 in gastric cancer tissues was lower than that in adjacent tissues. SLC7A11-AS1 expression was related to gross typing and TNM staging. Multivariate logistic regression analysis shown that the gastric cancer invasion were related to T stage, N stage and SLC7A11-AS1 expression level. The OS of patients with low expression of SLC7A11-AS1 (21. 78±3. 16) was lower than that of patients with high expression of SLC7A11-AS1 (34. 23±3. 48). The specificity of SLC7A11-AS1 tissue expression level for the diagnosis of gastric cancer was 82. 10% , and the sensitivity was 53. 80% (AUC=0. 69, P=0. 042). Conclusion SLC7A11- AS1 may be involved in the occurrence and development of gastric cancer as a tumor suppressor gene, especially in the process of invasion and migration of gastric cancer. Low expression of SLC7A11-AS1 can be used as a poor prognostic indicator, which provides an important reference for the diagnosis and prognosis of gastric cancer.