【Abstract】 Objective To explore the effects of thymosin β4 (Tβ4) combined with exogenous basic fibroblast growth factor (bFGF) on wound healing in rats with deep II degree scald. Methods 40 molding rats with deep II degree scald were randomly divided into normal control group (external of saline),β4 group (external application of 6 μg Tβ4) , bFGF group (external application of 4 μg bFGF) and Tβ4 + bFGF group (external application of 6 p.g β4 + 4 μg bFGF). On the 3rd, 7th, 10th and 14th d of administration, the wounds were photographed and the wound skin samples were taken. The wound healing rate in each group was calculated. The expression levels of TGF-β1 mRNA and VEGF mRNA were detected by RT-PCR. The protein expression levels of VEGF, Caspase-3 and NF-kB were detected by Western blot. On the 1st, 3rd, 6th, 12th, 24th and 48th h after administration, the wound skin samples of each group were taken, and the positive expression levels of IL-10 and TNF-a were detected by immunohistochemistry. Results At each time point after administration, the wound healing rate in Tβ4 + bFGF group was higher than that in the other groups (P＜0. 05) , and the expression level of TGF-βl mRNA was lower than that in the other groups (P＜0. 05) , while the expression level of VEGF mRNA and the protein expression levels of VEGF, Caspase-3 and NF-kB were higher than those in the other groups (P＜0. 05). The quantities of IL-10 and TNF-a positive cells in Tβ4 + bFGF group were increased first and then decreased, and peaked at 12 h, and the quantities were higher than those in β4 group and bFGF group before 12 h and were lower than those in Tβ4 group and bFGF group after 12 h (P＜0. 05). Conclusion β4 combined with exogenous bFGF can improve the inflammatory response, accelerate the apoptosis o£ necrotic tissue cells, promote the angiogenesis and accelerate wound healing for deep II degree scald.