【Abstract】 Objective To explore whether Muller cells can be transformed into neural stem cells after retinal injury, and can differentiate and regenerate many types o£ neurons in the retina after induction, and to explore whether the cultured cells have the ability o£ multi differentiation. Methods 72 female adult SD mouse were used to establish an optic nerve severing model. The retinal Muller cells o£ SD mouse were digested and purified by enzymatic hydrolysis. The 4th generation trypsin-digested retinal Muller cells were cultured for 5 days in modified Eagle medium (DMEM)-F12 medium containing basic fibroblast growth factor (FGF-2) and epidermal growth factor (EGF). The cells were differentiated by DMEM medium containing 0.5μmol/L RA, 1μ/L BDNF and 0.5% fetal bovine serum for 10 days, and the cells after dedi££erentiation and re-differentiation were identified by immunofluorescence staining. Results Immunofluorescence staining showed that Muller cells cultured in 3〜4 passages showed that the specific markers o£ glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) were positive, showing red color. The proportion o£ positive cells expressing fluorescent staining o£ GS and GLAST antibody was (92.76± 4.28) % and (95.28± 2.79) % , respectively. Immunofluorescence staining was performed on the dedifferentiated Muller cell sphere. The results showed that the specific marker nestin was positively expressed in red, and the proportion o£ fluorescent staining positive cells was (95.37± 1.46) %. Immunofluorescence staining was used to identify the dedifferentiated Muller cell spheres. The results showed that the glial cell-specific marker glial fibrillary acidic protein (GFAP) was positively expressed in green, and the proportion of fluorescent staining positive cells was(10.28 ±3.37) %. Some cells were positive for anti-5 bromo-2 deoxyuridine (BrdU) and showed red color. The proportion o£ positive cells stained with fluorescent staining was (90.44 ±4.23) %. The Muller cell spheres were induced by immunofluorescence staining. The results showed that the specific marker o£ Thyl. 1 was positive for ganglion cells and showed green color, mainly axon and cytoplasm. The proportion o£ fluorescent staining positive cells was (21.25 ± 4.62)%. Conclusion Retinal Muller cells are a potential source of retinal stem cells. Cytokine stimulation can induce stem cell characteristics in retinal Muller cells cultured in vitro.