【Abstract】 Objective To explore the regulatory effects of remifenil (Rem)-mediated AMPKal/PGC-1 a/GLUT4 pathway on blood lipids and inflammation in rates with diabetic atherosclerosis (AS). Methods 75 SD rats were randomly divided into control group (NC, 15 rats)and diabetic model group (DM, 60 rats). NC group was fed with normal diet, and DM group was fed with high-fat diet, and the DM rat model was established by intraperitoneal injection of streptozo- tocin (40 mg/kg). DM rats were randomly divided into DM group and high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups, with 15 rats in each group. High dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups were pumped with Rem for 5 min via tail vein for preprocessing (at rates of 1.6, 0.8 and 0.4 μg/kg/min respectively) , and were fed with high-fat diet for 12 w. The levels of TC, TG, HDL and LDL in serum were analyzed by biochemical analyzer. The levels of IL-6, IL-1R, iNOS and TNF-a in serum were determined by ELISA. The levels of LDH, MDA and SOD were detected by kit. Apoptosis of myocardial tissues was detected by TUNEL staining. Western Blot was used to detect the expression levels of ACC, SREBP-lc, CPT-1, PPARy, Caspase-3, Caspase-9, AMPKal, PGC-1 a and GLUT4. Results Compared with DM group, the levels of TC, TG,LDL, iNOS, IL-6, IL-1β and TNF-a in high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM- Rem groups were significantly decreased (P<0.05), the expression levels of ACC, SREBP-lc, Caspase-3 and Caspase-9 in high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups were significantly down- regulated (P<0.05), the apoptosis rate o£ myocardial tissues in high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups was significantly decreased (P<0.05), HDL level in high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups was increased significantly (P<0.05), the expression levels o£ CPT-1, PPARy, AMPKal, PGC-la and GLUT4 in high dose DM-Rem groups, medium dose DM-Rem groups and low dose DM-Rem groups were significantly up-regulated (P<0.05). Conclusion Rem may lower blood lipids and inflammatory factors and protect myocardial tissue damage by regulating the expression o£ AMPKal/PGC-la/GLUT4 signaling pathway.