【Abstract】 Objective lb establish the animal model of direct renal iryury by contrast medium, and provide the model reference for the study o£ contrast medium nephropathy. Methods SD rats were given iodized contrast media by water deprivation combined with intravenous administration. The better disease phenotype was obtained by detecting the changes o£ renal iryury markers and observing the changes o£ renal histomorphology and apoptosis. Results The serum creatinine and urea nitrogen levels were almost restored to the baseline value in the water control group after drinking water for 24 hours, but the serum creatinine level in the water control group was significantly increased （54. 45士 14. 39 μmol/L vs. 28. 26士9. 98 μmol/L； P＜0. 01） and the urea nitrogen level was also continuously increased （34. 70士2. 66 μmol/L vs. 10. 75士5. 45 μmol/L； P＜0. 001）. HE staining showed that the damage o£ the tissue in the model group was mainly concentrated in the proximal tubules at the junction of renal cortex and medulla and in the outer medulla, which showed vacuolar degeneration o£ tubule epithelial cells, flat atrophy or necrosis o£ cells. The difference o£ damage score was significant （2.0士0. 82 vs. 0.46士0.49； P=0.02）, At the same time, the expression of apoptotic protein Bax was significantly increased in group e (P= 0.02) and group f compared with the control group (P = 0.015). Apoptosis rate in group e (16.2 ± 5.2% vs. 5.1 ± 1.3%; P <0.01) and group f were significantly increased compared with the control group (20 ± 8.7% vs. 5.1 ± 1.3%; P <0.01).Conclusion Sing the comprehensive index analysis and evaluation, direct contrast agent contact combined with water deprivation pretreatment method can establish a stable contrast agent nephropathy model.