【Abstract】 Objective To investigate the expression of long-chain non coding RNA (LncRNA) in serum and cancer tissues of patients with laryngeal cancer and its relationship with prognosis. Methods 100 patients with laryngeal cancer diagnosed in our hospital from January 2013 to January 2018 were selected as the observation group. 60 healthy persons matched with age and sex in the observation group were selected as the control group. The expression level of serum LncRNA H19 in the two groups was determined by real-time fluorescent quantitative PCR (QRT PCR). The expressionof lncrna H19 in serum, cancer tissue and matched cancer tissue were detected. The observation group was divided into high expression group (55 cases) and low expression group (45 cases) according to the expression level of lncrnah19in cancer tissue, paracancerous tissue and serum. The relationship between the expression level of lncrnah19 in cancer tissue and the pathological characteristics and prognosis of patients was analyzed. Results The relative expression level of serum LncRNA 19 in the observation group was significantly higher than that in the control group (P＜0.05). The relative expression level of LncRNA H19 in cancer tissues was significantly higher than that in matched adjacent tissues(P＜0.05). There was a significant positive correlation between the relative expression level of LncRNA H19 in serum and cancer tissues in the observation group(P＜0.05). The proportion of TNM stage III, low differentiation and lymph node metastasis in the high expression group was significantly higher than that in the low expression group(P＜0.05). The total survival time and progression free survival time of patients in the high expression group of LncRNA H19 were significantly shorter than those in the low expression group(P＜0.05). Conclusion The expression of LncRNAH19 in the serum and tissues of patients with laryngeal cancer is upregulated. LncRNA may be involved in the process of carcinogenesis and progression of laryngeal cancer, which can be used as an effective index for prognosis evaluation of patients with laryngeal cancer.