【Abstract】 Objective To investigate the value of plasma heat shock protein 90 α (Hsp90 α) and squamous cell carcinoma antigen (SCC) in early diagnosis and prognosis monitoring of cervical cancer. Methods From April 2015 to June 2017, 96 patients with cervical cancer were selected as cervical cancer group. 50 cases of endometrial neoplasia in the same period were selected as the endometrial neoplasia group. 50 healthy women were taken as the control group. In the cervical cancer group, 2ml of EDTA K2 anticoagulant blood samples and accelerating blood samples were taken 3 days before operation, 3 days after operation and 1 week after operation. 2 ml of EDTA K2 anticoagulant blood samples were taken in the uteri intraepithelial neoplasia group and the normal control group, and stored in refrigerator at 80 ℃ after centrifugation. The SCC level was determined by chemiluminescence. The level of Hsp90 α was determined by ELISA. SCC was evaluated by receiver operating characteristic curve (ROC curve). The value of Hsp90 α level in the early diagnosis of cervical cancer and in the prognosis of patients with different tumor stages, differentiation, metastasis and tumor size were analyzed. Results The levels of Hsp90 α and SCC in cervical cancer group were higher than those in utero intraepithelial neoplasia group and normal control group, and the difference was statistically significant (P＜0.05). The diagnostic accuracy of SCC in cervical cancer was significantly lower than that of Hsp90 α (P＜0.05). The positive rate of Hsp90 α was higher than that of SCC (P＜0.05). After treatment, the levels of Hsp90 α and SCC decreased, and the difference was statistically significant (P＜0.05). In the follow-up of one year, 8 cases of cervical cancer recurred, the detection level of Hsp90 α was significantly higher than that of the non recurred patients, the difference was statistically significant (P＜0.05). Hsp90 α was related to clinical stage, differentiation, metastasis and tumor size (P＜0.05). SCC was related to the clinical stage of tumor (P＜0.05). Conclusion Plasma Hsp90 α and serum SCC can be used as the prediction indexes of cervical cancer, but the prediction effect of HSPPO is better than that of serum SCC. Plasma HSPPoa can be used as the reliable diagnosis index of cervical cancer, and can be used as the evaluation index of postoperative treatment effect, which is related to tumor stage, grade and metastasis. The combined detection of the two groups can reduce the misdiagnosis rate.