【Abstract】 Objective To investigate the effect of different doses of coffee on lipid metabolism in rats with nonalcoholic fatty liver disease.〖WTHZ〗Methods 80 6 week old SPF grade SD rats weighing (180 ± 20) g were randomly divided into blank group, model group, highdose coffee group and lowdose coffee group. The rats in the blank group were fed with normal diet and the rats in the other three groups were fed with highfat diet for 8 weeks. The high and low dose coffee group were given 60 and 20 mg / kg caffeine per day, and the blank group and the model group were given normal saline for 8 weeks. After 8 weeks, the body weight and liver index of rats were measured. The changes of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), R glutamate transferase (GGT), triglyceride (TG), total cholesterol (TC), highdensity lipoprotein cholesterol (HDL-C) and lowdensity lipoprotein cholesterol (LDL-C) were measured by automatic biochemical analyzer. HE staining was used to observe the pathological changes of liver. Results Compared with the blank group, the body weight, liver index, alt, AST, GGT, TG, TC and LDL-C contents of the model group increased significantly, while the HDL C content decreased significantly (P＜0.05). Compared with the model group, the liver index, alt, AST, GGT, TG, TC and LDL C contents of the rats treated with different doses of coffee decreased significantly, and the above indexes of the highdose coffee group were significantly lower than those of the lowdose Coffee Group (P＜0.05). Compared with the model group, the HDL C of different dose coffee group was significantly higher, and the HDL C of high dose coffee group was significantly higher than that of low dose Coffee Group (P＜0.05). In the model group, the tension of liver capsule was high and the margin was round. The edge of the liver of the rats treated with coffee was sharp. In the model group, the fatty change of hepatocytes was diffuse and vesicular, and the hepatic sinuses were narrowed obviously due to edema. However, the fatty infiltration of hepatocytes in the model group was significantly reduced after coffee treatment, and the cytoplasm of hepatocytes was uniform without edema. Conclusion Coffee can effectively alleviate the development of fatty liver in rats, reduce the inflammatory response in liver by regulating lipid metabolism, and regulate lipid metabolism in a concentration dependent range. Coffee can make the liver soft, elastic, sharp and neat.