【Abstract】 Objective To investigate the effect of traditional and electronic smoke on insulin sensitivity in ApoE knockout mice. Methods 48 male apoE knockout mice were randomly divided into ecigarette group (ecigarette group), ecigarette group without nicotine (0 mg group), traditional cigarette group (cigarette group) and control group (control group). Each group had 12 mice. 18 weeks′smoke poisoning experiment was carried out. The food intake, blood lipid, hypersensitive Creactive protein (HC CRP) and blood lipid were measured. The levels of tumor necrosis factor α (TNF-α) were measured by insulin tolerance test. Results Compared with control group, total cholesterol, triglyceride, LDL, HC CRP and TNF-α in ecigarette group, 0 mg group and cigarette group increased, but HDL decreased, with statistical significance (P＜0.05). Total cholesterol, triglyceride, LDL and HDL in cigarette group, ecigarette group and 0 mg group increased. There was no significant difference in the levels of Creactive protein and TNF-α between the two groups (P＞0.05). Compared with the control group, there was significant difference in the insulin tolerance test between the ecigarette group and the JP2 cigarette group (P＜0.05). Conclusion Both ecigarettes and traditional cigarettes can affect the metabolism of apoE knockout mice. The mechanism may be through the influence of lipid level and the induction of chronic inflammation, resulting in the decrease of insulin sensitivity in mice.