【Abstract】 Objective To investigate the mechanism of recombinant human interleukin11 (rhIL-11) promoting the invasion and metastasis of lung adenocarcinoma A549 cells by activating JAK/STAT3/HIF-1 α/EMT pathway. Methods Human lung adenocarcinoma A549 cells were divided into rhIL-11 0 μg/L Group (control group), rhIL-11 0μg/L group, rhIL-11 40 μ/l group, rhIL-11 80 μg/L group, rhIL 11 0 μg/L + AG490 (JAK inhibitor) group, rhIL 11 10 μ g/L+ AG490 group, rhIL 11 40μg/L + AG490 group and rhIL-11 80μg/L+ AG490 group. The effects of rhIL 11 on cell migration and invasion were studied by scratch test and Transwell invasion test. The mRNA and protein expression of STAT3, HIF 1 α, twist and E cadherin were detected by QRT PCR and Western blot. Results rhIL-11 promoted the migration and invasion of lung cancer A549 cells. Compared with the control group, the mRNA and protein expression of STAT3, HIF 1 α and twist genes in rhIL-11 80 μ g/L group increased significantly (P＜0.05), while the mRNA and protein expression of E cadherin gene decreased significantly (P＜0.05). Compared with rhIL-11 80 μg/L group, the mRNA and protein expression of STAT3, HIF 1-α and twist in rhIL-11 80 μg/L+AG490 group decreased significantly (P＜0.01). Conclusion rhIL-11 promotes the invasion and metastasis of lung cancer A549 cells, which may be due to the activation of JAK/STAT3/HIF 1 α/EMT signaling pathway.