【Abstract】 Objective The target genes of miRNA 200 family members were predicted and bioinformatics analyzed to provide evidence for further study of the function and regulatory mechanism of miRNA 200 family. Methods Target Scan, PicTar2 and Miranda were used to predict the target genes of the nine members of miR200 family, and then the intersection of the predicted target genes was obtained. Target genes were also predicted by miRTarbase, which were validated by at least three experiments and the type of targeting relationship was functional microRNAtarget gene interaction. The final target genes were combined with the results of Target Scan, PicTar2, Miranda, and miRTarbase. Bioinformatics function analysis including GeneOntology analysis, signal pathway analysis, and proteinprotein interaction (PPI) networks analysis were performed. Results The predicted target genes of miR 200 family were enriched in many biological processes, which were mainly enriched in transcriptional negative regulation of RNA polymerase Ⅱ promoter, DNA template transcriptional negative regulation, transcriptional regulation of RNA polymerase Ⅱ promoter, etc. The signal pathway was mainly enriched in tumor, glioma, melanoma, small cell lung cancer and other tumors, and infection related pathways such as EB virus infection and hepatitis B. The protein interaction network shown that there were complex interactions among the predicted target gene coding proteins of 9 members, and the target gene coding proteins "Rac1", "SRC", "RhoA", "crebbp" and "CTNNB1" played a key role in the interaction network. Conclusion Members of the Mir 200 family participate in the pathophysiological process of tumor and infection by regulating the target gene and then regulating the downstream target protein; Interaction pathway; Signal path