Objective To detect levels of peripheral serum procalcitonin (PCT), interleukin6 (IL-6), Creactive protein (CRP), amylase (AMY), and lipase (LPS) in patients with acute pancreatitis (AP) and analyze the value of combined detection of these five serum indicators in the clinical diagnosis of AP. Methods 117 patients with acute pancreatitis were enrolled in the hospital from January 2015 to January 2017, including 62 patients with severe acute pancreatitis (SAP) and 55 patients with mild acute pancreatitis (MAP). Another 110 healthy subjects were selected as the control group. The concentrations of PCT, IL-6, CRP, AMY and LPS in the serum of the SAP group, the MAP group and the control group were examined. The differences in serum PCT, IL-6, CRP, AMY and LPS levels were compared between groups. Using SPSS20.0 software for data analysis, the ROC curve was used to detect the serum value of the diagnosis of acute pancreatitis. Results The levels of PCT, IL-6, CRP, AMY and LPS in SAP group were significantly higher than those in the control group, and the difference was statistically significant (t values were 9.97, 12.95, 14.31, 15.38 and 8.83, P＜0.01). The five serum indexes of patients in the MAP group were significantly higher than those in the control group, and the difference was statistically significant (t values were 9.32, 13.59 and 11.97, 13.43 and 11.27, P＜0.01). The PCT, IL-6 and CRP levels were higher in the SAP group than in the MAP group. The difference was statisticallysignificant (tvalues were 4.43, 5.30 and 6.18, P＜0.05), and the difference in AMY and LPS betweenthe two groups. Not statistically significant (t values were 0.994 and 0.409, respectively, P＞0.05). The ROC curve areas of PCT, IL-6, CRP, AMY and LPS were 0.72, 0.87, 0.84, 0.88 and 0.86, and the sensitivity is 60.40%, 71.80%, 67.50%, 70.90% and 78.60%, and the specificity is 82.70%, 86.40%, 90.90%, 88.20%, 85.50%. The combined area of multiple serum indicators for the diagnosis of AP was 0.97, the sensitivity was 86.80%, and the specificity was 99.10%. Conclusion The detection of serum PCT, IL-6, CRP, AMY and LPS indicators has high sensitivity and specificity for the diagnosis of AP, which can effectively improve the clinical diagnosis accuracy of AP patients.