【Abstract】 Objective To study the diagnosis value gastroesophageal reflux disease (GRED) by gastric-filling ultrasound and endoscope. Methods 64 GERD patients in clinical final diagnosis were collected from March 2011 to September 2018. A retrospective analysis was conducted by comparing the results of gastroesophageal filling ultrasound with endoscope and summarized the characteristics of ultrasound images of GERD. The detection rate of the two methods and clinical diagnostic value were explored. Results In 64 GERD cases, 52 cases were detected by gastric filling ultrasound and positive rate was 81 % (52/64). 12 cases were not detected and negative rate was 18% (12/64). 31 cases were detected by endoscope and positive rate was 48% (31/64). 33 cases were not detected. The negative rate was 51% (33/64). The difference of detection rate in two ways was statistically significant. Conclusion Gastric filling ultrasound examination has strong operability with real-time observation of reflux vocal image. Compare with endoscopic diagnosis of GERD, gastric filling ultrasound is intuitive, simple, painless, no adverse reaction and high detection rate.