【Abstract】 Objective To investigate the effect of cassia glycosides on sterol regulatory element binding protein-lc (SREBP-lc) and peroxisome proliferators-activated receptor a (PPARa) in nonalcoholic fatty liver disease (NAFLD) rats. Methods 60 SD rats were randomly divided into the normal group, model group, positive drug polyene phosphatidylcholine group, cassia glycosides low, middle and high dose groups, n=10. Except the normal group, the others were receive a 8 weeks of diet with high fat and high sugar to build the NAFLD model, after the NAFLD model was successfully constructed, those rats were received a 6 weeks treatment, then the rats were sacrificed. Serum and liver were collected. Hepatic pathology was detected by HE staining, liver index was calculated, ALP, Tbil, DbiL HDL-C and LDLC were measured. Real-time PCR and Western Blot were used to determine the expression of SREBP-lc and PPARa protein and mRNA in liver tissue. Results Compared with the normal group , the results of HE staining showed obvious fat vacuoles the model group, while the polyene phosphatidylcholine and cassia glycoside could significantly reduce the num-ber of fat vacuoles. Compared with the normal group, the liver index, ALP, Tbil, Dbil, and LDL-C were significantly increased in model group (P<0.05). Compared with the model group, those indexes in the polyene phosphatidylcholine and cassia glycoside groups were significantly decreased (P<0.05). The liver index and ALP in the high dose of cassia glycoside group were significantly lower than those in the polyene phosphatidylcholine group (P<0.05). Tbil and Dbil in thethreedosegroupsofcassiaglycosidegroup weresignificantlylowerthanthoseinthepolyenephosphatidylcholine group (P<0.05). However , for LDL-C there was no difference between the polyene phosphatidylcholine and cassia glucosides group. Compared with the normal group , the HDL-C in the model group was significantly lower(P<0.05) , polyene phosphatidylcholine and cassia glucosides could increase HDL-C(P<0.05) , there were no significant differences between polyene phosphatidylcholine group and p cassia glycoside groups( After treatment with cassia glucosides , compared with the model group , SREBP-1c was significantly decreased(P<0.05) , PPAR# was significantly increased (P<0.05), and for them the cassia glycoside groups were significantly lower than polyene phosphatidylcholine group (P<0.05). Conclusion Compared with the commonly used drug polyene phosphatidylcholine in clinic , cassia glycoside can obviously improve liver function and regulate blood lipids , which is related with cassia glycoside could inhibition of SREBP-1c expression in liver.