【Abstract】 Objective To investigate the changes of metabolic profiles of serum free fatty acid (FFA) in patients with allergic rhinitis and find potential biomarkers for the diagnosis of allergic rhinitis. Methods Serum free fatty acid levels in 20 normal subjects (control group) and 20 patients with allergic rhinitis (case group) were measured by gas chromatographymass spectrometry (GC/MS) method. The principal component analysis (PCA) and partial least squares discriminant analysis (PLSDA) analysis were used to analyze changes in the profile of fatty acid metabolism in the patient's serum based on metabolomic analysis. Results The serum free fatty acid spectrum of allergic rhinitis group and normal control group had a good separation trend. By partial least squaresdiscriminant analysis, palmitic acid (C16:0), heptadecanoic acid (C17:0), stearic acid (C18:0), linoleic acid (C18:2), arachidic acid (C20:0), palmitic acid (C21: 0), cinereic acid (C22:0) were screened between allergic rhinitis group and normal control group, and the 7 fatty acids had significant differences between the groups (P＜0.01). The ROC curve was plotted for seven significantly different fatty acids, and the area under the ROC curve was calculated. The area under the C16:0 curve was 0.778, and the area under the C17:0 was 0.785,. The area under the C18:0 curve is 0.830, the area under the C18:2 curve is 0.72, the area under the C20:0 curve is 0.83, the area under the C21:0 curve is 0.83, and the area under the C22:0 curve is 0840. Conclusion The occurrence of allergic rhinitis is related to the disorder of lipid metabolism in the body, palmitic acid (C16:0), heptadecanoic acid (C17:0), stearic acid (C18:0), linoleic acid (C18:2), peanuts Acid (C20:0), palmitate (C21:0), citric acid (C22:0) are potential biomarkers for allergic rhinitis.